IGF1 Kit CLIA

N° du produit ABIN5655396

Détail du produit IGF1 Kit CLIA

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.

Sensibilité: 10.1 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor 1 (IGF1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: Insulin Like Growth Factor 1 (IGF1 Produits)

Synonymes: IGF-I Kit CLIA, IGF1A Kit CLIA, IGFI Kit CLIA, C730016P09Rik Kit CLIA, Igf-1 Kit CLIA, Igf-I Kit CLIA, Npt2B Kit CLIA, IGF-1 Kit CLIA, IGFIA Kit CLIA, IGF-IB Kit CLIA, igf1 Kit CLIA, IGF-1L Kit CLIA, IGF-1a Kit CLIA, IGF1 Kit CLIA, igf-1 Kit CLIA, igf1-A Kit CLIA, igf1.S Kit CLIA, xigf1 Kit CLIA, insulin like growth factor 1 Kit CLIA, insulin-like growth factor 1 Kit CLIA, insulin-like growth factor I Kit CLIA, insulin like growth factor 1 L homeolog Kit CLIA, IGF1 Kit CLIA, Igf1 Kit CLIA, LOC100136741 Kit CLIA, igf1 Kit CLIA, LOC678666 Kit CLIA, igf1.L Kit CLIA, LOC104911603 Kit CLIA

Sujet:

Gene Name: Insulin Like Growth Factor 1

Gene Aliases: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

ID gène: 3479

UniProt: P05019

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation