IGFBP4 Kit ELISA
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- Antigène Voir toutes IGFBP4 Kits ELISA
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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Reactivité
- Boeuf (Vache)
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 74.07 pg/mL - 6000 pg/mL
- Seuil minimal de détection
- 74.07 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.
- Sensibilité
- 29.62 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 h
- Plaque
- Pre-coated
- Protocole
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and unlabeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) (Standards or samples) with the pre-coated antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes IGFBP4 Kits ELISA
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- Autre désignation
- Insulin Like Growth Factor Binding Protein 4 (IGFBP4 Produits)
- Synonymes
- IGFBP4 Kit ELISA, igfbp4 Kit ELISA, BP-4 Kit ELISA, HT29-IGFBP Kit ELISA, IBP4 Kit ELISA, IGFBP-4 Kit ELISA, AI875747 Kit ELISA, Deb2 Kit ELISA, IGF-BP4 Kit ELISA, IBP-4 Kit ELISA, insulin like growth factor binding protein 4 Kit ELISA, insulin-like growth factor-binding protein 4 Kit ELISA, insulin-like growth factor binding protein 4 Kit ELISA, IGFBP4 Kit ELISA, LOC100136305 Kit ELISA, LOC100194498 Kit ELISA, igfbp4 Kit ELISA, Igfbp4 Kit ELISA
- Sujet
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Gene Name: Insulin Like Growth Factor Binding Protein 4
Gene Aliases: BP4, HT29-IGFBP, IBP4
- Pathways
- Signalisation WNT, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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