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JAM2 Kit ELISA

JAM2 Reactivité: Humain Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656138
  • Antigène Voir toutes JAM2 Kits ELISA
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Reactivité
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Junctional Adhesion Molecule 2 (JAM2). No significant cross-reactivity or interference between Junctional Adhesion Molecule 2 (JAM2) and analogues was observed.
    Sensibilité
    12.7 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junctional Adhesion Molecule 2 (JAM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junctional Adhesion Molecule 2 (JAM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junctional Adhesion Molecule 2 (JAM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Junctional Adhesion Molecule 2 (JAM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes JAM2 Kits ELISA
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Autre désignation
    Junctional Adhesion Molecule 2 (JAM2 Produits)
    Synonymes
    JAM2 Kit ELISA, cd322 Kit ELISA, jam-b Kit ELISA, jamb Kit ELISA, pro245 Kit ELISA, ve-jam Kit ELISA, vejam Kit ELISA, jam2 Kit ELISA, sb:cb1002 Kit ELISA, im:7155552 Kit ELISA, sc:d0813 Kit ELISA, si:ch211-160b11.5 Kit ELISA, LOC100227766 Kit ELISA, 1110002N23Rik Kit ELISA, 2410030G21Rik Kit ELISA, 2410167M24Rik Kit ELISA, JAM-2 Kit ELISA, JAM-B Kit ELISA, Jcam2 Kit ELISA, VE-JAM Kit ELISA, C21orf43 Kit ELISA, CD322 Kit ELISA, JAMB Kit ELISA, PRO245 Kit ELISA, VEJAM Kit ELISA, junctional adhesion molecule 2 Kit ELISA, junctional adhesion molecule 2a Kit ELISA, junctional adhesion molecule 2b Kit ELISA, junction adhesion molecule 2 Kit ELISA, JAM2 Kit ELISA, jam2 Kit ELISA, jam2a Kit ELISA, jam2b Kit ELISA, Jam2 Kit ELISA
    Sujet

    Gene Name: Junctional Adhesion Molecule 2

    Gene Aliases: CD322, JAM-B, JAMB, VE-JAM, VEJAM, Junctional adhesion molecule B, Vascular endothelial junction-associated molecule

    ID gène
    58494
    UniProt
    P57087
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