RAC1 Kit ELISA

N° du produit ABIN5658357

Détail du produit RAC1 Kit ELISA

Antigène: RAC1

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RAC1

Autre désignation: Ras Related C3 Botulinum Toxin Substrate 1 (RAC1 Produits)

Synonymes: Rac-1 Kit ELISA, TC-25 Kit ELISA, p21-Rac1 Kit ELISA, AL023026 Kit ELISA, D5Ertd559e Kit ELISA, RAC2 Kit ELISA, CRAC1A Kit ELISA, rac1 Kit ELISA, wu:fd16e02 Kit ELISA, zgc:55823 Kit ELISA, zgc:55917 Kit ELISA, zgc:86934 Kit ELISA, 2248 Kit ELISA, CG2248 Kit ELISA, D-Rac Kit ELISA, D-Rac 1 Kit ELISA, D-Rac1 Kit ELISA, DRac Kit ELISA, DRac1 Kit ELISA, DRacA Kit ELISA, Dm Rac1 Kit ELISA, DmRAC Kit ELISA, DmRAC1 Kit ELISA, Dmel\CG2248 Kit ELISA, Dmrac1 Kit ELISA, Drac Kit ELISA, Drac1 Kit ELISA, Drac1a Kit ELISA, DracA Kit ELISA, GTPase Rac1 Kit ELISA, P40792 Kit ELISA, RAC1 Kit ELISA, Rac Kit ELISA, Rac GTPase Kit ELISA, Rac3 Kit ELISA, RacA Kit ELISA, dRac Kit ELISA, dRac1 Kit ELISA, drac1 Kit ELISA, rac Kit ELISA, Xrac Kit ELISA, mig5 Kit ELISA, p21-rac1 Kit ELISA, tc-25 Kit ELISA, Rac family small GTPase 1 Kit ELISA, RAS-related C3 botulinum substrate 1 Kit ELISA, ras-related C3 botulinum toxin substrate 1 Kit ELISA, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Kit ELISA, Rac family small GTPase 1a Kit ELISA, CG2248 gene product from transcript CG2248-RB Kit ELISA, Rac family small GTPase 1 L homeolog Kit ELISA, ras homolog family member G Kit ELISA, RAC1 Kit ELISA, Rac1 Kit ELISA, rac1a Kit ELISA, rac1.L Kit ELISA, RHOG Kit ELISA

Sujet:

Gene Name: Ras Related C3 Botulinum Toxin Substrate 1

Gene Aliases: TC-25, p21-Rac1, MIG5, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1

ID gène: 5879

UniProt: P63000

Pathways: Signalisation WNT, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling