SMUG1 Kit ELISA

N° du produit ABIN5658805

Détail du produit SMUG1 Kit ELISA

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). No significant cross-reactivity or interference between Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Autre désignation: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1 Produits)

Synonymes: SMUG1 Kit ELISA, xsmug1 Kit ELISA, 1200013B09Rik Kit ELISA, A930006H09Rik Kit ELISA, C85220 Kit ELISA, FDG Kit ELISA, HMUDG Kit ELISA, UNG3 Kit ELISA, single-strand-selective monofunctional uracil-DNA glycosylase 1 Kit ELISA, single-strand selective monofunctional uracil DNA glycosylase Kit ELISA, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog Kit ELISA, SMUG1 Kit ELISA, smug1 Kit ELISA, smuG1 Kit ELISA, smuG Kit ELISA, LOC5577584 Kit ELISA, CpipJ_CPIJ002767 Kit ELISA, Smug1 Kit ELISA, smug1.L Kit ELISA

Sujet:

Gene Name: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1

Gene Aliases: FDG, HMUDG, UNG3

ID gène: 23583

UniProt: Q53HV7

Pathways: Réparation de l'ADN