KIT Ligand Kit CLIA

N° du produit ABIN5658948

Détail du produit KIT Ligand Kit CLIA

Antigène: KIT Ligand (KITLG)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor (SCF). No significant cross-reactivity or interference between Stem Cell Factor (SCF) and analogues was observed.

Sensibilité: 1.22 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor (SCF) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: Stem Cell Factor (KITLG Produits)

Synonymes: Xkl-1 Kit CLIA, Xsl Kit CLIA, Xsl-1 Kit CLIA, Xsl-2 Kit CLIA, steel Kit CLIA, KITLG Kit CLIA, SCF Kit CLIA, kitl Kit CLIA, kl-1 Kit CLIA, mgf Kit CLIA, scf Kit CLIA, FPH2 Kit CLIA, KL-1 Kit CLIA, Kitl Kit CLIA, MGF Kit CLIA, SF Kit CLIA, SHEP7 Kit CLIA, Clo Kit CLIA, Con Kit CLIA, Gb Kit CLIA, Kitlg Kit CLIA, Mgf Kit CLIA, SLF Kit CLIA, Sl Kit CLIA, blz Kit CLIA, contrasted Kit CLIA, 710-712 Kit CLIA, CSF Kit CLIA, KITL Kit CLIA, KIT ligand L homeolog Kit CLIA, KIT ligand Kit CLIA, kit ligand Kit CLIA, kitlg.L Kit CLIA, KITLG Kit CLIA, kitlg Kit CLIA, Kitl Kit CLIA, Kitlg Kit CLIA

Sujet:

Gene Name: Stem Cell Factor

Gene Aliases: KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway