Survivin Kit ELISA

N° du produit ABIN5659043

Détail du produit Survivin Kit ELISA

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Survivin (Surv). No significant cross-reactivity or interference between Survivin (Surv) and analogues was observed.

Sensibilité: 5.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Survivin (Surv). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Survivin (Surv). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Survivin (Surv), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Survivin (Surv) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Survivin (Surv) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Survivin (Surv) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: Survivin (BIRC5 Produits)

Synonymes: AP14 Kit ELISA, API4 Kit ELISA, EPR-1 Kit ELISA, AAC-11 Kit ELISA, Api4 Kit ELISA, TIAP Kit ELISA, survivin40 Kit ELISA, bir5a Kit ELISA, survivin Kit ELISA, BIR1 Kit ELISA, Su2 Kit ELISA, XBIR1 Kit ELISA, XSurvivin1 Kit ELISA, Xsvv1 Kit ELISA, api4 Kit ELISA, bir1-A Kit ELISA, epr-1 Kit ELISA, svv1 Kit ELISA, xSurvivin Kit ELISA, xsurv Kit ELISA, Su1 Kit ELISA, birc5.2 Kit ELISA, svv2 Kit ELISA, xSurvivin2 Kit ELISA, bir5b Kit ELISA, SIX Kit ELISA, birc5.2-a Kit ELISA, birc5.2-b Kit ELISA, baculoviral IAP repeat containing 5 Kit ELISA, baculoviral IAP repeat-containing 5 Kit ELISA, baculoviral IAP repeat containing 5a Kit ELISA, baculoviral IAP repeat-containing 5, gene 1 L homeolog Kit ELISA, baculoviral IAP repeat containing 5 L homeolog Kit ELISA, baculoviral IAP repeat containing 5b Kit ELISA, baculoviral IAP repeat containing 5 S homeolog Kit ELISA, BIRC5 Kit ELISA, Birc5 Kit ELISA, birc5a Kit ELISA, birc5.1.L Kit ELISA, birc5.2.L Kit ELISA, birc5b Kit ELISA, birc5.2.S Kit ELISA

Sujet:

Gene Name: Survivin

Gene Aliases: BIRC5, API4, EPR-1, Baculoviral Inhibitor Of Apoptosis Repeat Containing 5, Apoptosis Inhibitor 4, Survivin Variant 3 Alpha

ID gène: 395937

UniProt: O57340

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding