TGFA Kit ELISA

N° du produit ABIN5659419

Détail du produit TGFA Kit ELISA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Alpha (TGFa). No significant cross-reactivity or interference between Transforming Growth Factor Alpha (TGFa) and analogues was observed.

Sensibilité: 3.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Alpha (TGFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Alpha (TGFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Alpha (TGFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: Transforming Growth Factor Alpha (TGFA Produits)

Synonymes: TFGA Kit ELISA, LOC100009482 Kit ELISA, TGF alpha Kit ELISA, TGFA Kit ELISA, wa-1 Kit ELISA, wa1 Kit ELISA, RATTGFAA Kit ELISA, TGFAA Kit ELISA, NV15606 Kit ELISA, si:ch211-10e8.7 Kit ELISA, transforming growth factor alpha Kit ELISA, transforming growth factor alpha L homeolog Kit ELISA, transforming growth factor-alpha Kit ELISA, transforming growth factor, alpha Kit ELISA, TGFA Kit ELISA, TGF-A Kit ELISA, Tgfa Kit ELISA, tgfa.L Kit ELISA, LOC100121300 Kit ELISA, LOC100009150 Kit ELISA, tgfa Kit ELISA

Sujet:

Gene Name: Transforming Growth Factor Alpha

Gene Aliases: ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF

ID gène: 24827

UniProt: P01134

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway