XBP1 Kit CLIA

N° du produit ABIN5660046

Détail du produit XBP1 Kit CLIA

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of X-Box Binding Protein 1 (XBP1). No significant cross-reactivity or interference between X-Box Binding Protein 1 (XBP1) and analogues was observed.

Sensibilité: 5.19 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Box Binding Protein 1 (XBP1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Autre désignation: X-Box Binding Protein 1 (XBP1 Produits)

Synonymes: treb5 Kit CLIA, xXBP1 Kit CLIA, xbp2 Kit CLIA, XBP1P1 Kit CLIA, XBPP1 Kit CLIA, xbp1 Kit CLIA, TREB5 Kit CLIA, XBP-1 Kit CLIA, XBP2 Kit CLIA, D11Ertd39e Kit CLIA, TREB-5 Kit CLIA, HTF Kit CLIA, cb918 Kit CLIA, id:ibd1195 Kit CLIA, trebf Kit CLIA, wu:fb02h05 Kit CLIA, wu:fb63f10 Kit CLIA, xbp1a Kit CLIA, xbp1b Kit CLIA, X-box binding protein 1 Kit CLIA, X-box binding protein 1 L homeolog Kit CLIA, xbp1 Kit CLIA, XBP1 Kit CLIA, Xbp1 Kit CLIA, xbp1.L Kit CLIA

Sujet:

Gene Name: X-Box Binding Protein 1

Gene Aliases: TREB5, XBP2, Tax-responsive element-binding protein 5

Pathways: ER-Nucleus Signaling, Unfolded Protein Response