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Apolipoprotein C-II Kit ELISA

APOC2 Reactivité: Souris Colorimetric Sandwich ELISA 7.8125 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674657
  • Antigène Voir toutes Apolipoprotein C-II (APOC2) Kits ELISA
    Apolipoprotein C-II (APOC2)
    Reactivité
    • 7
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    • 2
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.8125 ng/mL - 500 ng/mL
    Seuil minimal de détection
    7.8125 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    3.3 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes Apolipoprotein C-II (APOC2) Kits ELISA
    Apolipoprotein C-II (APOC2)
    Autre désignation
    Apolipoprotein C2 (APOC2) (APOC2 Produits)
    Synonymes
    apoc2 Kit ELISA, apoC-II Kit ELISA, fb98g08 Kit ELISA, wu:fb52e03 Kit ELISA, wu:fb71a04 Kit ELISA, wu:fb98g08 Kit ELISA, APOC2 Kit ELISA, APO-CII Kit ELISA, APOC-II Kit ELISA, RGD1560725 Kit ELISA, APOCII Kit ELISA, Apo-CII Kit ELISA, ApoC-II Kit ELISA, apolipoprotein C-II Kit ELISA, apolipoprotein C2 Kit ELISA, apoc2 Kit ELISA, APOC2 Kit ELISA, Apoc2 Kit ELISA
    Sujet
    Alternative name: APC2, Apo-C2, ProapoC-II, Apolipoprotein C-II, Proapolipoprotein C-II
    ID gène
    11813
    UniProt
    Q05020
    Pathways
    Lipid Metabolism
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