PARP1 Kit ELISA

N° du produit ABIN5690733

Détail du produit PARP1 Kit ELISA

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Human Cleaved PARP (D214/G215) Cell-based ELISA Kit. This ELISA is for measuring cleaved PARP (Asp-214/Gly-215) in human adherent cell lines.

Type d'échantillon: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human PARP cleaved at sites aspartic acid-214 and glycine-215.

Attributs du produit:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Ingrédients:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Uncoated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP (PARP1 Produits)

Synonymes: ADPRT Kit ELISA, ADPRT 1 Kit ELISA, ADPRT1 Kit ELISA, ARTD1 Kit ELISA, PARP Kit ELISA, PARP-1 Kit ELISA, PPOL Kit ELISA, pADPRT-1 Kit ELISA, Adprt Kit ELISA, Parp-1 Kit ELISA, 5830444G22Rik Kit ELISA, AI893648 Kit ELISA, Adprp Kit ELISA, Adprt1 Kit ELISA, C80510 Kit ELISA, parp-1 Kit ELISA, sPARP-1 Kit ELISA, BEST:LD21673 Kit ELISA, CG17685 Kit ELISA, CG17696 Kit ELISA, CG17718 Kit ELISA, CG40411 Kit ELISA, D.PARP Kit ELISA, Dm.pARTa Kit ELISA, Dmel\\CG40411 Kit ELISA, LD21673.3prime Kit ELISA, PARP1 Kit ELISA, dPARP Kit ELISA, parp Kit ELISA, adprt1 Kit ELISA, padprt-1 Kit ELISA, ppol Kit ELISA, si:dkey-206f10.3 Kit ELISA, wu:fc60f12 Kit ELISA, zgc:110092 Kit ELISA, poly(ADP-ribose) polymerase 1 Kit ELISA, poly (ADP-ribose) polymerase 1 Kit ELISA, poly (ADP-ribose) polymerase family, member 1 Kit ELISA, Poly-(ADP-ribose) polymerase Kit ELISA, poly(ADP-ribose) polymerase 1 L homeolog Kit ELISA, PARP1 Kit ELISA, Parp1 Kit ELISA, Parp Kit ELISA, parp1.L Kit ELISA, parp1 Kit ELISA

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location