Caspase 3 Kit ELISA
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- Antigène Voir toutes Caspase 3 (CASP3) Kits ELISA
- Caspase 3 (CASP3)
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Reactivité
- Humain, Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Cell ELISA
- Application
- ELISA
- Fonction
- CASP-3 (D175) Cell-based ELISA Kit. This ELISA is for measuring cleaved CASP-3 (Asp-175) in human adherent cell lines.
- Type d'échantillon
- Adherent Cell Culture
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-3 cleaved at site Aspartic Acid-175
- Attributs du produit
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- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
- Ingrédients
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- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- Matériel non inclus
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Plaque
- Uncoated
- Protocole
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Caspase 3 (CASP3) Kits ELISA
- Caspase 3 (CASP3)
- Autre désignation
- Caspase-3 (CASP3 Produits)
- Synonymes
- CPP32 Kit ELISA, CPP32B Kit ELISA, SCA-1 Kit ELISA, A830040C14Rik Kit ELISA, AC-3 Kit ELISA, Apopain Kit ELISA, CC3 Kit ELISA, Caspase-3 Kit ELISA, Lice Kit ELISA, Yama Kit ELISA, mldy Kit ELISA, xcpp32 Kit ELISA, casp3 Kit ELISA, zgc:100890 Kit ELISA, CASP-3 Kit ELISA, caspase-3 Kit ELISA, caspase 3 Kit ELISA, caspase 3 S homeolog Kit ELISA, caspase 3, apoptosis-related cysteine peptidase a Kit ELISA, caspase 3, apoptosis-related cysteine peptidase Kit ELISA, CASP3 Kit ELISA, Casp3 Kit ELISA, casp3.S Kit ELISA, casp3a Kit ELISA
- ID gène
- 836
- UniProt
- P42574
- Pathways
- Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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