IL12A Kit ELISA

N° du produit ABIN5692238

Détail du produit IL12A Kit ELISA

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Épitope: AA 23-215, AA 23-335

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-12(P70). 96wells/kit, with removable strips.

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21, Immunogen sequence: (M23-S335)+(R23-S215)

Sensibilité: < 1.5 pg/mL

Ingrédients: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Information d'application

Durée du test: 15 min

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Date de péremption: 12 months

Détail du antigène

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Autre désignation: Il12a (IL12A Produits)

Synonymes: CLMF Kit ELISA, IL-12A Kit ELISA, NFSK Kit ELISA, NKSF1 Kit ELISA, P35 Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12p35 Kit ELISA, Il-12a Kit ELISA, Ll12a Kit ELISA, p35 Kit ELISA, IL12p35 Kit ELISA, Il-12 p35 Kit ELISA, IL12p40 Kit ELISA, IL12 Kit ELISA, IL-12 p35 Kit ELISA, interleukin 12A Kit ELISA, interleukin 12B Kit ELISA, interleukin 12b Kit ELISA, interleukin 12a Kit ELISA, Interleukin 12 level Kit ELISA, IL12A Kit ELISA, IL12B Kit ELISA, Il12b Kit ELISA, Il12a Kit ELISA, IL12 Kit ELISA, il12a Kit ELISA

Sujet:

Synonyms: Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a

Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Cellular Localisation: Secreted.

UniProt: Q9R103

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation