IL12A Kit ELISA (Interleukin 12 alpha)

Details for Product IL12A ELISA Kit No. ABIN411310, Fournisseur: Connectez-vous pour afficher
Antigène
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • Il12a
  • IL12A
Épitope
AA 23-335, AA 23-215
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
16
12
11
7
6
6
4
4
3
2
2
2
1
1
1
Type de méthode
Sandwich ELISA
Gamme de detection
15.6-1000 pg/mL
Seuil minimal de détection
15.6 pg/mL
Application
ELISA
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Fonction Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-12(p70)
Marque PicoKine™
Type d'échantillon Cell Culture Supernatant, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Immunogène Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)
Specificité Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)
Réactivité croisée (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensibilité <1.5pg/mL
Matériel non inclus Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmids, Primers & others IL12A products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation IL12A (IL12A ELISA Kit Extrait)
Sujet

Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Synonyms: Interleukin-12 subunit alpha ,Il12a ,

Full Gene Name: interleukin 12A

ID gène 16159
UniProt F8WI71
Pathways Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Indications d'application Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plaque Pre-coated
Protocole mouse IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(P70) amount of sample captured in plate.
Procédure de l'essai

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(P70) standard solution and each sample be measured in duplicate.

Précision du teste
  • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 6.5, CV(%): 6.7
  • Sample 2: n=16, Mean(pg/ml): 367, Standard deviation: 25.7, CV(%): 7
  • Sample 3: n=16, Mean(pg/ml): 633, Standard deviation: 34.2, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 123, Standard deviation: 9.23, CV(%): 7.5
  • Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 33.05, CV(%): 8.1
  • Sample 3: n=24, Mean(pg/ml): 657, Standard deviation: 38.8, CV(%): 5.9
Restrictions For Research Use only
Conseil sur la manipulation Avoid multiple freeze-thaw cycles.
Stock -20 °C,4 °C
Stockage commentaire Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Date de péremption 12 months
Images (Fournisseur)
ELISA image for Interleukin 12 alpha (IL12A) ELISA Kit (ABIN411310) Mouse IL-12(p70) PicoKine ELISA Kit standard curve
Produit citée dans: Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." dans: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014 (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." dans: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014 (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." dans: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014 (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." dans: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014 (PubMed).

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." dans: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013 (PubMed).

Huang, Yang, Jiang, Shao, Sun, Chen, Dong, Zhang: "Anti-tumor immune responses of tumor-associated macrophages via toll-like receptor 4 triggered by cationic polymers." dans: Biomaterials, Vol. 34, Issue 3, pp. 746-55, 2012 (PubMed).

Huang, Zhang, Jiang, Zhang, Chen, Dong, Zhang: "Targeted delivery of oligonucleotides into tumor-associated macrophages for cancer immunotherapy." dans: Journal of controlled release : official journal of the Controlled Release Society, Vol. 158, Issue 2, pp. 286-92, 2012 (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." dans: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012 (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." dans: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012 (PubMed).

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." dans: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011 (PubMed).

Background publications Shi, Chen, Wang, Zhu, Xu, Wang, Xiao, Shi, Zhang, Xu: "Short amyloid-beta immunogens show strong immunogenicity and avoid stimulating pro-inflammatory pathways in bone marrow-derived dendritic cells from C57BL/6J mice in vitro." dans: Peptides, Vol. 32, Issue 8, pp. 1617-25, 2011 (PubMed).

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