Interferon gamma Kit ELISA
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- Antigène Voir toutes Interferon gamma (IFNG) Kits ELISA
- Interferon gamma (IFNG)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15-15000 pg/mL
- Seuil minimal de détection
- 15 pg/mL
- Application
- ELISA
- Fonction
- Human IFN-gamma ELISA Kit for cell and tissue lysate samples.
- Type d'échantillon
- Tissue Lysate, Cell Lysate
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes human IFN-gamma.
- Sensibilité
- 15 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Cell lysate buffer
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Interferon gamma (IFNG) Kits ELISA
- Interferon gamma (IFNG)
- Autre désignation
- IFN-gamma (IFNG Produits)
- Synonymes
- IFG Kit ELISA, IFI Kit ELISA, IFN-g Kit ELISA, Ifg Kit ELISA, IFNG2 Kit ELISA, IFN-gamma Kit ELISA, IFN-G Kit ELISA, IFNG Kit ELISA, IFNgamma Kit ELISA, TCRalpha Kit ELISA, INF-G Kit ELISA, ifng Kit ELISA, interferon gamma Kit ELISA, interferon, gamma 1-2 Kit ELISA, IFNG Kit ELISA, Ifng Kit ELISA, ifng1-2 Kit ELISA
- Sujet
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Gene Names: IFNG
Protein names: Interferon gamma (IFN-gamma) (Immune interferon)
- ID gène
- 3458
- UniProt
- P01579
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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