TYRO3 Kit ELISA

N° du produit ABIN625289

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain TYRO3.

Aperçu rapide pour TYRO3 Kit ELISA (ABIN625289)

Antigène: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-10,000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit TYRO3 Kit ELISA

Seuil minimal de détection: 40 pg/mL

Fonction: Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Attributs du produit: Custom Human Dtk ELISA Kit available on demand

Ingrédients: Pre-Coated 96-Well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Matériel non inclus: Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Autre désignation: Dtk

Pathways: Signalisation RTK