XIAP Kit ELISA

N° du produit ABIN625470

Détail du produit XIAP Kit ELISA

Antigène: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.25-600 ng/mL

Seuil minimal de détection: 0.25 ng/mL

Application: ELISA

Fonction: Human XIAP ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair can recognize human and mouse XIAP.

Sensibilité: 250 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Autre désignation: XIAP (XIAP Produits)

Synonymes: API3 Kit ELISA, BIRC4 Kit ELISA, IAP-3 Kit ELISA, ILP1 Kit ELISA, MIHA Kit ELISA, XLP2 Kit ELISA, hIAP-3 Kit ELISA, hIAP3 Kit ELISA, api3 Kit ELISA, birc4 Kit ELISA, ilp1 Kit ELISA, miha Kit ELISA, xlp2 Kit ELISA, xxiap Kit ELISA, 1110015C02Rik Kit ELISA, Aipa Kit ELISA, Api3 Kit ELISA, Birc4 Kit ELISA, IAP3 Kit ELISA, ILP-1 Kit ELISA, mXIAP Kit ELISA, riap3 Kit ELISA, fb73d01 Kit ELISA, wu:fb73d01 Kit ELISA, zgc:91873 Kit ELISA, BIRC8 Kit ELISA, X-linked inhibitor of apoptosis Kit ELISA, X-linked inhibitor of apoptosis L homeolog Kit ELISA, XIAP Kit ELISA, xiap Kit ELISA, xiap.L Kit ELISA, Xiap Kit ELISA

Sujet: Gene Names: XIAP API3 BIRC4 IAP3
Protein names: E3 ubiquitin-protein ligase XIAP (EC 6.3.2.-) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (X-linked inhibitor of apoptosis protein) (X-linked IAP)

ID gène: 331

UniProt: P98170

Pathways: Apoptose, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis