CD80 Kit ELISA
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- Antigène Voir toutes CD80 Kits ELISA
- CD80
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5 pg/mL - 4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: Activation B7-1 antigen, B7, BB1, CD80, CD80_HUMAN, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD82
Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Gene Name: CD80
Production: Natural and recombinant rat CD80. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: I39-Q248
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. It is recommended that each rat CD80 standard solution and each sample be measured in duplicate.
ELISA Dilution: 62.5pg/mL-4000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes CD80 Kits ELISA
- CD80
- Autre désignation
- CD80 - B7-1 (CD80 Produits)
- Synonymes
- B7 Kit ELISA, B7-1 Kit ELISA, B7.1 Kit ELISA, BB1 Kit ELISA, CD28LG Kit ELISA, CD28LG1 Kit ELISA, LAB7 Kit ELISA, B71 Kit ELISA, Cd28l Kit ELISA, Ly-53 Kit ELISA, Ly53 Kit ELISA, MIC17 Kit ELISA, TSA1 Kit ELISA, CD80 molecule Kit ELISA, Cd80 molecule Kit ELISA, CD80 antigen Kit ELISA, CD80 Kit ELISA, Cd80 Kit ELISA
- ID gène
- 25408
- NCBI Accession
- NP_037058
- UniProt
- O35187
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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