KIT Ligand Kit ELISA

N° du produit ABIN6719754

Détail du produit KIT Ligand Kit ELISA

Antigène: KIT Ligand (KITLG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: C kit ligand, Ckit ligand, Kit ligand, Kitl, KITLG, KL 1, KL1, Mast cell growth factor, MGF, SF, SHEP7, Steel factor, Stem cell factor, Stem cell factor precursor

Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

Gene Name: KITLG

Production: Natural and recombinant human SCF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: E26-A190

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human SCF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA, citrate) to each empty well. It is recommended that each human SCF standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: SCF (KITLG Produits)

Synonymes: Xkl-1 Kit ELISA, Xsl Kit ELISA, Xsl-1 Kit ELISA, Xsl-2 Kit ELISA, steel Kit ELISA, KITLG Kit ELISA, SCF Kit ELISA, kitl Kit ELISA, kl-1 Kit ELISA, mgf Kit ELISA, scf Kit ELISA, FPH2 Kit ELISA, KL-1 Kit ELISA, Kitl Kit ELISA, MGF Kit ELISA, SF Kit ELISA, SHEP7 Kit ELISA, Clo Kit ELISA, Con Kit ELISA, Gb Kit ELISA, Kitlg Kit ELISA, Mgf Kit ELISA, SLF Kit ELISA, Sl Kit ELISA, blz Kit ELISA, contrasted Kit ELISA, 710-712 Kit ELISA, CSF Kit ELISA, KITL Kit ELISA, KIT ligand L homeolog Kit ELISA, KIT ligand Kit ELISA, kit ligand Kit ELISA, kitlg.L Kit ELISA, KITLG Kit ELISA, kitlg Kit ELISA, Kitl Kit ELISA, Kitlg Kit ELISA

ID gène: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway