CD25 Kit ELISA

N° du produit ABIN6719914

N° du produit (Fournisseur): koa0217

Kit ELISA Humain CD25, test Colorimetric pour la quantification de Humain CD25.

Aperçu rapide pour CD25 Kit ELISA (ABIN6719914)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CD25 Kit ELISA

Seuil minimal de détection: 78 pg/mL

N° du produit (Fournisseur): koa0217

Fournisseur: Rockland

Fonction: Human CD25 - IL-2sR alpha Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CD25. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each human CD25 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: E22-C213

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25/IL-2sR alpha

Sujet:

Synonyms: CD122, CD122 antigen, CD132, CD132 antigen, CD25, CD25 antigen, Cytokine receptor common gamma chain, Gamma C, High affinity IL 2 receptor subunit beta, IL 2 RA, IL 2 receptor, IL 2 receptor alpha subunit, IL 2R gamma chain, IL2 RA, IL2R IL2RA, IL2RB, IL2RG, IMD4, interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, interleukin 2 receptor beta, Interleukin 2 receptor beta chain, interleukin 2 receptor gamma, Interleukin 2 receptor gamma chain, TAC antigen

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

ID gène: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation