IL-7 Kit ELISA

N° du produit ABIN6719939

N° du produit (Fournisseur): koa0459

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain IL-7.

Aperçu rapide pour IL-7 Kit ELISA (ABIN6719939)

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Serum

Détail du produit IL-7 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0459

Fournisseur: Rockland

Fonction: Human IL-7 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IL-7. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human IL-7 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: D26-H177

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: IL-7

Sujet:

Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin7

Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.

ID gène: 3574

NCBI Accession: NP_000871

UniProt: A8K673

Pathways: Signalistation JAK/STAT