IGF1R Kit ELISA

N° du produit ABIN6720107

N° du produit (Fournisseur): koa0457

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain IGF1R.

Aperçu rapide pour IGF1R Kit ELISA (ABIN6720107)

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit IGF1R Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0457

Fournisseur: Rockland

Fonction: Human IGF1R Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IGF1R. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGF1R standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: E31-N932

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: IGF1R

Sujet:

Synonyms: CD221, IGF 1R, IGF I receptor, IGF-I receptor, Igf1r, IGF1R_HUMAN, IGFIR, IGFR, Insulin like growth factor 1 receptor, Insulin like growth factor I receptor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216

Background: IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

ID gène: 3480

NCBI Accession: NP_000866

UniProt: P08069

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy