IGFBP4 Kit ELISA

N° du produit ABIN6720208

Détail du produit IGFBP4 Kit ELISA

Antigène: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 500 pg/mL - 32000 pg/mL

Seuil minimal de détection: 500 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AI875747, BP 4, BP4, Deb2, HT29 IGFBP, IBP 4, IBP-4, IBP4, IBP4_HUMAN, IGF binding protein 4, IGF-binding protein 4, IGF-BP4, IGFBP 4, IGFBP-4, IGFBP4, Insulin Like Growth Factor Binding Protein 4, Insulin-like growth factor-binding protein 4

Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

Gene Name: IGFBP4

Production: Natural and recombinant human IGFBP4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D22-E258

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 32000pg/mL, 16000pg/mL, 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL human IGFBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGFBP4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 500pg/mL-32000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Autre désignation: IGFBP4 (IGFBP4 Produits)

Synonymes: IGFBP4 Kit ELISA, igfbp4 Kit ELISA, BP-4 Kit ELISA, HT29-IGFBP Kit ELISA, IBP4 Kit ELISA, IGFBP-4 Kit ELISA, AI875747 Kit ELISA, Deb2 Kit ELISA, IGF-BP4 Kit ELISA, IBP-4 Kit ELISA, insulin like growth factor binding protein 4 Kit ELISA, insulin-like growth factor-binding protein 4 Kit ELISA, insulin-like growth factor binding protein 4 Kit ELISA, IGFBP4 Kit ELISA, LOC100136305 Kit ELISA, LOC100194498 Kit ELISA, igfbp4 Kit ELISA, Igfbp4 Kit ELISA

ID gène: 3487

NCBI Accession: NP_001543

UniProt: P22692

Pathways: Signalisation WNT, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process