NGFR Kit ELISA

N° du produit ABIN6720237

Détail du produit NGFR Kit ELISA

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD271, CD271 antigen, Gp80 LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Nerve growth factor receptor, Nerve growth factor receptor TNFR superfamily member 16, NGF receptor, NGFR, p75 ICD, p75 ICD, p75 Neurotrophin receptor, p75NTR, TNFR Superfamily Member 16, TNFRSF16, TNR16_HUMAN, Tumor necrosis factor receptor superfamily member 16

Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.

Gene Name: NGFR

Production: Natural and recombinant human TNFRSF16. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: K29-N250

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: TNFRSF16 - NGFR (NGFR Produits)

Synonymes: ngfr Kit ELISA, wu:fb38c02 Kit ELISA, wu:fc10h01 Kit ELISA, CD271 Kit ELISA, Gp80-LNGFR Kit ELISA, TNFRSF16 Kit ELISA, p75(NTR) Kit ELISA, p75NTR Kit ELISA, LNGFR Kit ELISA, RNNGFRR Kit ELISA, Tnfrsf16 Kit ELISA, p75 Kit ELISA, NRH1b Kit ELISA, fullback Kit ELISA, nrh1 Kit ELISA, p75ntr Kit ELISA, tnfrsf16 Kit ELISA, p75NGFR Kit ELISA, nerve growth factor receptor Kit ELISA, neurotrophin receptor associated death domain Kit ELISA, nerve growth factor receptor S homeolog Kit ELISA, nerve growth factor receptor (TNFR superfamily, member 16) Kit ELISA, NGFR Kit ELISA, nradd Kit ELISA, Ngfr Kit ELISA, ngfr.S Kit ELISA

ID gène: 4804

NCBI Accession: NP_002498

UniProt: P08138

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding