ErbB2/Her2 Kit ELISA

N° du produit ABIN6720274

Détail du produit ErbB2/Her2 Kit ELISA

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD340, CD340 antigen, Cerb B2/neu protein, CerbB, HER2, Her2/neu, Herstatin, Metastatic lymph node gene 19 protein, MLN19, NEU, NEU Proto Oncogene, Neuro Glioblastoma Derived Oncogene Homolog, NGL, Proto oncogene c ErbB 2, Proto oncogene Neu, Receptor protein tyrosine kinase ErbB2 precursor, Receptor tyrosine protein kinase erbB 2, TKR1, Tyrosine kinase type cell surface receptor HER2, v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog

Background: HER2/neu(also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.

Gene Name: ERBB2

Production: Natural and recombinant human ErbB-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T23-T652

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ErbB-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin, EDTA) or tissue lysates to each empty well. It is recommended that each human ErbB-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Autre désignation: ErbB-2 (ErbB2/Her2 Produits)

Synonymes: CD340 Kit ELISA, HER-2 Kit ELISA, HER-2/neu Kit ELISA, HER2 Kit ELISA, MLN 19 Kit ELISA, NEU Kit ELISA, NGL Kit ELISA, TKR1 Kit ELISA, Erbb-2 Kit ELISA, Neu Kit ELISA, c-erbB2 Kit ELISA, c-neu Kit ELISA, mKIAA3023 Kit ELISA, wu:fv70f10 Kit ELISA, zgc:63601 Kit ELISA, erb2 Kit ELISA, erb-b2 receptor tyrosine kinase 2 Kit ELISA, ERBB2 Kit ELISA, Erbb2 Kit ELISA, erbb2 Kit ELISA

ID gène: 2064

NCBI Accession: NP_001005862

UniProt: P04626

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development