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Syndecan 1 Kit ELISA

SDC1 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730295
  • Antigène Voir toutes Syndecan 1 (SDC1) Kits ELISA
    Syndecan 1 (SDC1)
    Reactivité
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse Syndecan-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse Syndecan-1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SDC1 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Syndecan 1 (SDC1) Kits ELISA
    Syndecan 1 (SDC1)
    Autre désignation
    Syndecan-1 (SDC1 Produits)
    Synonymes
    CG10497 Kit ELISA, CT27296 Kit ELISA, Dmel\\CG10497 Kit ELISA, Dsyn Kit ELISA, Syd Kit ELISA, Syn Kit ELISA, dSdc Kit ELISA, l(2)10608 Kit ELISA, sdc Kit ELISA, syd Kit ELISA, syn Kit ELISA, Syn1 Kit ELISA, cd138 Kit ELISA, synd1 Kit ELISA, xSyn1 Kit ELISA, syndecan1 Kit ELISA, Syndecan-1 Kit ELISA, XSyndecan-1 Kit ELISA, SDC1 Kit ELISA, CD138 Kit ELISA, SDC Kit ELISA, SYND1 Kit ELISA, syndecan Kit ELISA, AA408134 Kit ELISA, AA409076 Kit ELISA, Sstn Kit ELISA, Synd Kit ELISA, Synd1 Kit ELISA, syn-1 Kit ELISA, HSPG Kit ELISA, SYNDECA Kit ELISA, Syndecan Kit ELISA, Syndecan Kit ELISA, syndecan 1 Kit ELISA, Sdc Kit ELISA, sdc1 Kit ELISA, SDC1 Kit ELISA, Sdc1 Kit ELISA
    UniProt
    P18828
    Pathways
    Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism
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