WISP1 Kit ELISA
WISP1
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951247
-
-
Antigène
Voir toutes WISP1 Kits ELISA
-
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
Reactivité
-
Souris
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Mouse WISP-1 ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product WISP1 Kit ELISA
-
-
-
Indications d'application
-
Optimal working dilution should be determined by the investigator.
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Date de péremption
-
6 months
-
-
Antigène
Voir toutes WISP1 Kits ELISA
-
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
Autre désignation
-
WISP-1 (WISP1 Produits)
-
Synonymes
-
CCN4 Kit ELISA, WISP1c Kit ELISA, WISP1i Kit ELISA, WISP1tc Kit ELISA, WISP-1 Kit ELISA, WISP1 Kit ELISA, si:ch211-38f10.2 Kit ELISA, AW146261 Kit ELISA, Elm1 Kit ELISA, zgc:172276 Kit ELISA, WNT1 inducible signaling pathway protein 1 Kit ELISA, WNT1 inducible signaling pathway protein 1a Kit ELISA, WNT1-inducible-signaling pathway protein 1-like Kit ELISA, WNT1 inducible signaling pathway protein 1b Kit ELISA, WISP1 Kit ELISA, wisp1a Kit ELISA, Wisp1 Kit ELISA, LOC100627044 Kit ELISA, wisp1b Kit ELISA
-
ID gène
-
22402
-
UniProt
-
O54775
-
Pathways
-
Signalisation WNT, Growth Factor Binding
-