CDK1 Kit ELISA

N° du produit ABIN6951405

Détail du produit CDK1 Kit ELISA

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Gallus CDK1/CDC2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDK1 (CDC2 (CDK1 Produits)

Synonymes: CDC2 Kit ELISA, CDC28A Kit ELISA, P34CDC2 Kit ELISA, Cdc2 Kit ELISA, Cdc2a Kit ELISA, p34 Kit ELISA, cdc2 Kit ELISA, wu:fc30e01 Kit ELISA, zgc:92032 Kit ELISA, PSTAIR Kit ELISA, cdc-2 Kit ELISA, cdc2-a Kit ELISA, cdc28a Kit ELISA, cdc2x1.1 Kit ELISA, p34cdc2 Kit ELISA, xcdc2 Kit ELISA, 5363 Kit ELISA, CDCDm Kit ELISA, CDK1 Kit ELISA, CDK1/CDC2 Kit ELISA, CG5363 Kit ELISA, Cdk-1 Kit ELISA, Cdk1 Kit ELISA, Dcdc2 Kit ELISA, Dm cdc2 Kit ELISA, DmCdc2 Kit ELISA, DmCdk1 Kit ELISA, Dmcdc2 Kit ELISA, Dmel\\CG5363 Kit ELISA, cdc Kit ELISA, cdc2Dm Kit ELISA, cdk1 Kit ELISA, dCdk1 Kit ELISA, group 4 Kit ELISA, l(2)31Eh Kit ELISA, CDC2A Kit ELISA, CDC2AAT Kit ELISA, CDK2 Kit ELISA, CDKA1 Kit ELISA, CDKA;1 Kit ELISA, CYCLIN-DEPENDENT KINASE A;1 Kit ELISA, cell division control 2 Kit ELISA, cdc2-b Kit ELISA, cdc2a Kit ELISA, cdc2x1.2 Kit ELISA, POL3 Kit ELISA, pold Kit ELISA, cyclin dependent kinase 1 Kit ELISA, cyclin dependent kinase like 1 Kit ELISA, Cell division control protein 2 1 Kit ELISA, cyclin-dependent kinase 1 Kit ELISA, cyclin-dependent kinase 1 S homeolog Kit ELISA, Cyclin-dependent kinase 1 Kit ELISA, cell division control 2 Kit ELISA, cell division control protein2 homolog Kit ELISA, cyclin-dependent kinase 1 L homeolog Kit ELISA, polymerase (DNA directed), delta 1, catalytic subunit L homeolog Kit ELISA, cyclin-dependent protein kinase Cdk1/Cdc2 Kit ELISA, CDK1 Kit ELISA, CDKL1 Kit ELISA, POPTR_0004s14080g Kit ELISA, Cdk1 Kit ELISA, cdk1 Kit ELISA, cdk1.S Kit ELISA, CDC2 Kit ELISA, cdc2 Kit ELISA, cdk-1 Kit ELISA, cdk1.L Kit ELISA, pold1.L Kit ELISA

ID gène: 396252

UniProt: P13863

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA