Survivin Kit ELISA

N° du produit ABIN6951658

Détail du produit Survivin Kit ELISA

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Survivin/BIRC5 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: Survivin (BIRC5 (BIRC5 Produits)

Synonymes: AP14 Kit ELISA, API4 Kit ELISA, EPR-1 Kit ELISA, AAC-11 Kit ELISA, Api4 Kit ELISA, TIAP Kit ELISA, survivin40 Kit ELISA, bir5a Kit ELISA, survivin Kit ELISA, BIR1 Kit ELISA, Su2 Kit ELISA, XBIR1 Kit ELISA, XSurvivin1 Kit ELISA, Xsvv1 Kit ELISA, api4 Kit ELISA, bir1-A Kit ELISA, epr-1 Kit ELISA, svv1 Kit ELISA, xSurvivin Kit ELISA, xsurv Kit ELISA, Su1 Kit ELISA, birc5.2 Kit ELISA, svv2 Kit ELISA, xSurvivin2 Kit ELISA, bir5b Kit ELISA, SIX Kit ELISA, birc5.2-a Kit ELISA, birc5.2-b Kit ELISA, baculoviral IAP repeat containing 5 Kit ELISA, baculoviral IAP repeat-containing 5 Kit ELISA, baculoviral IAP repeat containing 5a Kit ELISA, baculoviral IAP repeat-containing 5, gene 1 L homeolog Kit ELISA, baculoviral IAP repeat containing 5 L homeolog Kit ELISA, baculoviral IAP repeat containing 5b Kit ELISA, baculoviral IAP repeat containing 5 S homeolog Kit ELISA, BIRC5 Kit ELISA, Birc5 Kit ELISA, birc5a Kit ELISA, birc5.1.L Kit ELISA, birc5.2.L Kit ELISA, birc5b Kit ELISA, birc5.2.S Kit ELISA

ID gène: 332

UniProt: O15392

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding