PRAS40 Kit ELISA

N° du produit ABIN7830405

Le kit ELISA PRAS40 (ABIN7830405) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PRAS40.

Aperçu rapide pour PRAS40 Kit ELISA (ABIN7830405)

Antigène: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit PRAS40 Kit ELISA

Fonction: AKT1S1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKT1S1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Autre désignation: AKT1S1

Sujet:

Synonyms: Lobe|MGC2865|PRAS40

Gene Description: AKT1 substrate 1 (proline-rich)

ID gène: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, L'effet Warburg