ARAF Kit ELISA

N° du produit ABIN7830421

Kit ELISA ARAF Humain, Colorimetric test pour la quantification de Humain ARAF.

Aperçu rapide pour ARAF Kit ELISA (ABIN7830421)

Antigène: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit ARAF Kit ELISA

Fonction: ARAF (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ARAF expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Autre désignation: ARAF

Sujet:

Synonyms: A-RAF|ARAF1|PKS2|RAFA1

Gene Description: v-raf murine sarcoma 3611 viral oncogene homolog

ID gène: 369

UniProt: P10398

Pathways: Signalisation MAPK, Signalisation RTK, Hepatitis C