ASK1 Kit ELISA

N° du produit ABIN7830424

Kit ELISA ASK1 Humain, Colorimetric test pour la quantification de Humain ASK1.

Aperçu rapide pour ASK1 Kit ELISA (ABIN7830424)

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit ASK1 Kit ELISA

Fonction: MAP3K5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAP3K5 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Autre désignation: MAP3K5

Sujet:

Synonyms: ASK1|MAPKKK5|MEKK5

Gene Description: mitogen-activated protein kinase kinase kinase 5

ID gène: 4217

UniProt: Q99683

Pathways: Signalisation MAPK, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response