Bcl-2 Kit ELISA

N° du produit ABIN7830439

Kit ELISA Bcl-2 Humain, Colorimetric test pour la quantification de Humain Bcl-2.

Aperçu rapide pour Bcl-2 Kit ELISA (ABIN7830439)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Bcl-2 Kit ELISA

Fonction: BCL2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BCL2 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: BCL2

Sujet:

Synonyms: Bcl-2

Gene Description: B-cell CLL/lymphoma 2

ID gène: 596

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling