CREB1 Kit ELISA

N° du produit ABIN7830525

Le kit ELISA CREB1 (ABIN7830525) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CREB1.

Aperçu rapide pour CREB1 Kit ELISA (ABIN7830525)

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit CREB1 Kit ELISA

Fonction: CREB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CREB1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Autre désignation: CREB1

Sujet:

Synonyms: CREB|MGC9284

Gene Description: cAMP responsive element binding protein 1

ID gène: 1385

UniProt: P16220

Pathways: Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation