Cyclin A1 Kit ELISA

N° du produit ABIN7830537

Le kit ELISA Cyclin A1 (ABIN7830537) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Cyclin A1.

Aperçu rapide pour Cyclin A1 Kit ELISA (ABIN7830537)

Antigène: Cyclin A1 (CCNA1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Cyclin A1 Kit ELISA

Fonction: CCNA1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNA1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Cyclin A1 (CCNA1)

Autre désignation: CCNA1

Sujet:

Synonyms: -

Gene Description: cyclin A1

ID gène: 8900

UniProt: P78396

Pathways: Apoptose, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA