CD25 Kit ELISA

N° du produit ABIN7830603

Le kit ELISA CD25 (ABIN7830603) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CD25.

Aperçu rapide pour CD25 Kit ELISA (ABIN7830603)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit CD25 Kit ELISA

Fonction: IL2RA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL2RA expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: IL2RA

Sujet:

Synonyms: CD25|IDDM10|IL2R|TCGFR

Gene Description: interleukin 2 receptor, alpha

ID gène: 3559

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation