E2F1 Kit ELISA

N° du produit ABIN7830610

Kit ELISA E2F1 Humain, Colorimetric test pour la quantification de Humain E2F1.

Aperçu rapide pour E2F1 Kit ELISA (ABIN7830610)

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit E2F1 Kit ELISA

Fonction: E2F1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of E2F1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F1

Sujet:

Synonyms: E2F-1|RBAP1|RBBP3|RBP3

Gene Description: E2F transcription factor 1

ID gène: 1869

UniProt: Q01094

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy