CDKN1B Kit ELISA
Aperçu rapide pour CDKN1B Kit ELISA (ABIN7830789)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- CDKN1B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDKN1B expression in cultured cells.
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Analytical Method
- Qualitative
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Autre désignation
- CDKN1B
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Sujet
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Synonyms: CDKN4|KIP1|MEN1B|MEN4|P27KIP1
Gene Description: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
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ID gène
- 1027
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Pathways
- Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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