p300 Kit ELISA

N° du produit ABIN7830797

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain p300.

Aperçu rapide pour p300 Kit ELISA (ABIN7830797)

Antigène: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit p300 Kit ELISA

Fonction: EP300 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EP300 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: p300 (EP300) (E1A Binding Protein P300 (EP300))

Autre désignation: EP300

Sujet:

Synonyms: KAT3B|p300

Gene Description: E1A binding protein p300

ID gène: 2033

Pathways: Signalisation p53, Signalisation Notch, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size