PPARG Kit ELISA
Aperçu rapide pour PPARG Kit ELISA (ABIN7830849)
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Voir toutes PPARG Kits ELISAReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- PPARG (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PPARG expression in cultured cells.
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Analytical Method
- Qualitative
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Réactivité croisée
- Souris, Rat
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Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA Kit
PPARG Reactivité: Humain Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Humain Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Souris Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Lapin Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Rat Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Autre désignation
- PPARG
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Sujet
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Synonyms: CIMT1|NR1C3|PPARG1|PPARG2|PPARgamma
Gene Description: peroxisome proliferator-activated receptor gamma
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ID gène
- 5468
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Pathways
- Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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