RPS6KB2 Kit ELISA
Aperçu rapide pour RPS6KB2 Kit ELISA (ABIN7830884)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- RPS6KB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KB2 expression in cultured cells.
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Analytical Method
- Qualitative
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Réactivité croisée
- Souris
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))
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Autre désignation
- RPS6KB2
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Sujet
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Synonyms: KLS|P70-beta|P70-beta-1|P70-beta-2|S6K-beta2|S6K2|SRK|STK14B|p70(S6K)-beta|p70S6Kb
Gene Description: ribosomal protein S6 kinase, 70 kDa, polypeptide 2
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ID gène
- 6199
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Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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