TGFB1 Kit ELISA
Aperçu rapide pour TGFB1 Kit ELISA (ABIN7830913)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- TGFB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TGFB1 expression in cultured cells.
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Analytical Method
- Qualitative
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Réactivité croisée
- Souris, Rat
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
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Autre désignation
- TGFB1
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Sujet
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Synonyms: CED|DPD1|TGFB|TGFbeta
Gene Description: transforming growth factor, beta 1
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ID gène
- 7040
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Pathways
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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