Kallikrein 12 Kit ELISA

N° du produit ABIN7832968

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Kallikrein 12.

Aperçu rapide pour Kallikrein 12 Kit ELISA (ABIN7832968)

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit Kallikrein 12 Kit ELISA

Seuil minimal de détection: 15.6 ng/mL

Fonction: Sandwich ELISA for quantitative detection of Human Kallikrein 12 concentrations.

Analytical Method: Quantitative

Sensibilité: 7140 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Autre désignation: KLK12

Sujet: Alternative Names: KLKL5, Kallikrein-12, Kallikrein-like protein 5, KLK-L5

ID gène: 43849

UniProt: Q9UKR0

Pathways: Système du Complément