TRADD Kit ELISA

N° du produit ABIN8056598

Kit ELISA TRADD Humain, Colorimetric test pour la quantification de Humain TRADD.

Aperçu rapide pour TRADD Kit ELISA (ABIN8056598)

Antigène: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Détail du produit TRADD Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: ELISA Kit for TNFRSF1A Associated Via Death Domain Protein (TRADD)

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of TNFRSF1A Associated Via Death Domain Protein (TRADD).
No significant cross-reactivity or interference between TNFRSF1A Associated Via Death Domain Protein (TRADD) and analogues was observed.

Sensibilité: < 0.055 ng/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 ± 10nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFRSF1A Associated Via Death Domain Protein (TRADD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFRSF1A Associated Via Death Domain Protein (TRADD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Autre désignation: TNFRSF1A Associated Via Death Domain Protein

Sujet: Tumor Necrosis Factor Receptor Type 1-Associated DEATH Domain Protein, TNFRSF1A-associated via death domain

UniProt: Q15628

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Hepatitis C