PRKAA1 Kit ELISA

N° du produit ABIN8070559

Le kit ELISA PRKAA1 (ABIN8070559) Humain, Souris et Rat est un kit ELISA Colorimetric conçu pour quantifier Humain, Souris et Rat PRKAA1.

Aperçu rapide pour PRKAA1 Kit ELISA (ABIN8070559)

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Épitope: pThr183, total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Lysate

Détail du produit PRKAA1 Kit ELISA

Fonction: Phospho-AMPKa1 (T183) and Total AMPKa1 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human, mouse, rat cell lysates.

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse and rat AMPK subunit alpha-1 phosphorylated at site Threonine-183.

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Positive Control
• Anti-Phospho Detection Antibody
• Anti-Pan Detection Antibody (*not included in phospho-only version)
• HRP-Conjugated Secondary Antibody
• HRP-Streptavidin (*not included in phospho-only version)
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: AMPKa1

Sujet:

Gene Symbols: PRKAA1, AMPK1

Protein Name (Synonyms): 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1), Acetyl-CoA carboxylase kinase (ACACA kinase), Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase), Tau-protein kinase PRKAA1

ID gène: 556210578765248

UniProt: Q13131, Q5EG47, P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg