Cyclin D1 Kit ELISA

N° du produit ABIN8077711

Kit ELISA Cyclin D1 Rat, Colorimetric test pour la quantification de Rat Cyclin D1.

Aperçu rapide pour Cyclin D1 Kit ELISA (ABIN8077711)

Antigène: Cyclin D1 (CCND1)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Détail du produit Cyclin D1 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: Rat Cyclin D1 (CCND1) ELISA Kit for detecting CCND1 in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensibilité: 0.064 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1

Sujet: Gene Aliases: CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene

ID gène: 58919

UniProt: P39948

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling