Leptin Kit ELISA
Aperçu rapide pour Leptin Kit ELISA (ABIN8082784)
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Voir toutes Leptin (LEP) Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.312 ng/mL
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Fonction
- Chicken Leptin (LEP) ELISA Kit for detecting LEP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensibilité
- 0.127 ng/mL
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Ingrédients
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Matériel non inclus
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Leptin (LEP) ELISA Kit
LEP Reactivité: Humain Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Humain Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reactivité: Lapin Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Souris Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Lapin Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivité: Poulet Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 h
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Plaque
- Pre-coated
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Précision du teste
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- 4°C for one month, Long term: see manual.
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Date de péremption
- 12 months
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- Leptin (LEP)
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Autre désignation
- Leptin
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Sujet
- Gene Aliases: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
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UniProt
- O42164
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Pathways
- Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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