PRKAA1 Kit ELISA

N° du produit ABIN8085884

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Rat PRKAA1.

Aperçu rapide pour PRKAA1 Kit ELISA (ABIN8085884)

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit PRKAA1 Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Rat Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) ELISA Kit for detecting AMPK Alpha 1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensibilité: 0.55 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: Protein Kinase, AMP Activated Alpha 1

Sujet: Gene Aliases: PRKAa1, AMPK, AMPKa1, PKA, ACACA kinase, 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase

ID gène: 65248

UniProt: P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg