IGF1 Kit ELISA

N° du produit ABIN8090598

Le kit ELISA IGF1 (ABIN8090598) Chien est un kit ELISA Colorimetric conçu pour quantifier Chien IGF1.

Aperçu rapide pour IGF1 Kit ELISA (ABIN8090598)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.563 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit IGF1 Kit ELISA

Seuil minimal de détection: 1.563 ng/mL

Fonction: Canine IGF-1 (Insulin-like growth factor I ) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with IGF-1 , no obvious cross reaction with other analogues.

Sensibilité: 0.938 ng/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Volume d'échantillon: 100 μL

Durée du test: 4 h

Plaque: Pre-coated

Protocole: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF-1

Sujet: Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, IGF1, IBP1

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation