Interferon gamma Kit ELISA

N° du produit ABIN8126237

Kit ELISA Interferon gamma Humain, Colorimetric test pour la quantification de Humain Interferon gamma.

Aperçu rapide pour Interferon gamma Kit ELISA (ABIN8126237)

Antigène: Interferon gamma (IFNG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Interferon gamma Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human IF-gamma antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IFN-gamma detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFN-gamma amount of samples captured in the plate.,This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 9.4 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human IFN-gamma concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL  

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human IF-gamma antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IFN-gamma detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFN-gamma amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IFN-gamma can be calculated using the standard curve. 

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: Interferon gamma (IFNG)

Autre désignation: IFN-Gamma (Interferon Gamma)

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy